Server.Execute

I am trying to deploy an ASP.Net (.Net Framework 1.1) on a Win2k3 Server (IIS6). If, in IIS, 'Enable Anonymous' - is ticked, the application works fine. However, I need to only permit Windows authentication. The application fails with an Error 401. This appears to occur at the point that the Server.Execute method is called pointing to another aspx page in the web application. I believe the configuration settings I am using in the web.config file are correct. In testing, I am allowing '*'. Also the NTFS permissions for testing are set to allow everyone for the Web application and the C:\WINDOWS\Microsoft.NET\Framework\v1.1.4322\Temporary ASP.NET Files folder.
Any ideas welcome. Thanks Grant

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